An equation was formulated that enabled the calculation of the concentration of free Ca2+ in a reaction medium containing calcium, magnesium (or manganese), and up to five metal chelators. The concentrations of all ionic and chelating species present in the incubation mixture could also be estimated
The effects of Ca2+ and ADP on the activity of NAD-linked isocitrate dehydrogenase of muscle
โ Scribed by H. Vaughan; E.A. Newsholme
- Book ID
- 115901137
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 261 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0014-5793
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## Abstract Cellโfree extracts of the obligate methanolโutilizing bacterium __Pseudomonas__ W6 catalyze the oxydation of isocitrate to ฮฑโketoglutarate in the presence of NAD^+^ and NADP^+^. After electroโfocusing of the crude extract of __Pseudomonas__ W6 actually two distinct bands each of NAD^+^โ