The effects of buffer diffusion on the activity of an immobilized esterase
โ Scribed by J. Konecny; J. Slanicka
- Book ID
- 115733842
- Publisher
- Elsevier Science
- Year
- 1975
- Weight
- 306 KB
- Volume
- 403
- Category
- Article
- ISSN
- 0005-2744
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
In order to further our understanding of immobilized enzyme reaction kinetics, the effects of external diffusion on the kinetic constants are studied for various reaction systems. It is shown that the variations of apparent kinetic constants with diftusional limitations are not the same as those of
## Abstract The effect of internal diffusion on the slope and the intercept of the LineweaverBurk plots for the immobilized enzyme was considered theoretically and it was found that the slope and the intercept are influenced not only by the dimensionless term __M__ but also by the range of the dime