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The effects of agitation intensity with and without continuous sparging on the growth and antibody production of hybridoma cells

✍ Scribed by Oh, S.K.W.; Nienow, A.W.; Al-Rubeai, M.; Emery, A.N.


Book ID
122827427
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
1010 KB
Volume
12
Category
Article
ISSN
0168-1656

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To investigate the response of hybridoma cells to hypoosmotic stress, S3H5/β₯2bA2 and DB9G8 hybridomas were cultivated in the hypoosmolar medium [Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% serum] resulting from sodium chloride subtraction. Both hybridomas showed similar responses