Chemical cleavage of mismatch: A new loo
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Timothy P. Ellis; Karen E. Humphrey; Margaret J. Smith; Richard G. H. Cotton
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Article
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1998
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John Wiley and Sons
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English
β 168 KB
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Chemical cleavage of mismatch (CCM), also known as chemical mismatch cleavage (CMC) or the HOT (hydroxylamine/osmium tetroxide) chemical method, has been used for detection of sequence variability with many systems since it was first described. Recently, adaptation to fluorescence-based detection sy