The effect of vinca alkaloids in enhancing the sensitivity of a methotrexate-resistant (L1210/R7A) line, studied by flow cytometric and chromosome number analysis

The use of a fluorescent methotrexate pr

The use of a fluorescent methotrexate probe to monitor the effects of three vinca alkaloids on a mixed population of parental L1210 and gene-amplified methotrexate-resistant cells by flow cytometry

✍ David G. Poppitt; Alan T. McGown; Brian W. Fox 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 374 KB

## Cells res&tant to methotrexate (LI210/R7A) and possessing an increased level of dihydrofolate reductase due to gene amplification can be detected by the technique of flow cytofluorimetry using a new fluorescent derivative of methotrexate (F-MTX) based on a putrescine linker. Comparative studies o