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The Effect of Tension on Closely Spaced Nicks in Naked DNA Molecules

โœ Scribed by Chung, Wei-Ju; Cui, Yujia; Hsu, Ian C.


Book ID
122133901
Publisher
Biophysical Society
Year
2012
Tongue
English
Weight
65 KB
Volume
102
Category
Article
ISSN
0006-3495

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The role of closely spaced lesions on both DNA strands in the induction of double-strand breaks and formation of deletions was studied. For this purpose a polylinker sequence flanked by 165 bp direct repeats was inserted within the tet gene of pBR327. This plasmid was used to construct DNA containin