The effect of temperature on the radial distribution function for iron in native horse spleen ferritin

✍ Scribed by K.R. Bauchspieß; T.G.St. Pierre; J. Webb

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 149 KB
• Volume: 208-209
• Category: Article
• ISSN: 0921-4526
• DOI: 10.1016/0921-4526(94)00875-v

✦ Synopsis

Iron K-edge X-ray absorption spectra were recorded for a sample of freeze-dried horse spleen ferritin over a range of temperatures from 40 to 300 K. Gaussian-type radial distribution functions were fitted to the data from all temperatures simultaneously and the obtained mean-square relative displacements a 2 (EXAFS Debye-Waller factors) were fitted using an Einstein model. For iron atoms in the second and/or third coordination shell an Einstein temperature of 330 + 20 K was obtained. If oxygen was assumed in the second or third shell, its Einstein temperature was 460 + 20 K. This indicates that the core of ferritin may have a somewhat more rigid structure than those of previously studied ferritin analogues (polysaccharide iron complexes).