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The effect of temperature on plasmid stability during the production of a heterologous protein by Escherichia coli in steady state conditions

✍ Scribed by F. Vázquez; D. Mattanovich; A. Villaverde; E. Rodriguez-Carmona


Publisher
Elsevier
Year
2009
Tongue
English
Weight
97 KB
Volume
25
Category
Article
ISSN
1871-6784

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## Abstract At the laboratory scale, sudden step increases from 30 to 42°C can be readily accomplished when expressing heterologous proteins in heat‐inducible systems. However, for large scale‐cultures only slow ramp‐type increases in temperature are possible due to heat transfer limitations, where