Newly synthesised DNA molecules the same size as replicons (7 million-60 million daltons) accumulate in yeast cells treated with hydroxyurea. During prolonged incubation in low concentrations of the drug, there is a large accumulation of these molecules without any corresponding increase in their mo
The effect of sulfite on the yeastSaccharomyces cerevisiae
β Scribed by Karl-Ludwig Schimz
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 884 KB
- Volume
- 125
- Category
- Article
- ISSN
- 0302-8933
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β¦ Synopsis
After a short period of tolerance, living cells of Saccharomyces cerevisiae were irreversibly damaged by low concentrations of sulfite. The length of the period of tolerance and the rate of the damaging effect depended on the concentration on sulfite, pH-value, temperature, the physiological state of the cells, and incubation time.
Inhibitors of protein synthesis and mitochondrial ATP synthesis did not alter the deleterious effect of sulfite on living cells. Furthermore, cell damage leading to inhibition of colony formation occured under aerobic as well as under anaerobic conditions.
Prior to cell inactivation sulfite induced the formation of respiratory deficient cells.
The active agent was shown to be SO2.
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