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The effect of sperm and cryoprotectant concentration on the freezing success of sex sorted ram sperm for in vitro fertilization

✍ Scribed by K.H. Beilby; S.P. de Graaf; C.G. Grupen

Book ID
116912261
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
643 KB
Volume
74
Category
Article
ISSN
0093-691X

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## Abstract Rat epididymal spermatozoa, at a concentration of 40–65 Γ— 10^4^ cells/ml, were preincubated for 5–5.5 hr and then added to eggs from superovu‐lated, immature rats. By 10–12 hr after insemination, 86% of the eggs had undergone fertilization, but many of them (41%) were polyspermic. When