In a preparation of soluble components from isolated spinach (Spinecia oleracea L.) chloroplasts, the activity of ribulose-l,5-bisphosphate carboxylase (EC 4.1.1.39) is strongly increased by 6-phosphogluconate or by NADPH at pH 8.0. When the thylakoid system is added to these soluble components (rec
The effect of SO3--on the activity of ribulose-1,5-diphosphate carboxylase in isolated spinach chloroplasts
โ Scribed by Irmgard Ziegler
- Publisher
- Springer-Verlag
- Year
- 1972
- Tongue
- English
- Weight
- 433 KB
- Volume
- 103
- Category
- Article
- ISSN
- 0032-0935
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โฆ Synopsis
S03--inhibits the activity of ribulose-l,5-diphosphate carboxylase in isolated spinach chloroplasts. It shows a non-competitive inhibition pattern with respect to ribulose-l,5-diphosphate and Mg++ but a competitive one with respect to I-ICOa-. The Ki-values are 14 mM S03--and 9.5 mM SOa--respectively for the non-competitive inhibition but only 3.0 mM S03--in the case of competitive inhibition with HC03--as a substrate. Thus it is concluded that the competitive inhibition type will predominate at low SO~--and low internal C02 concentrations.
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