✦ LIBER ✦

The effect of sample composition and vial type on Ĉerenkov counting in a liquid scintillation counter

✍ Scribed by Thomas F. Kellogg

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 522 KB
Volume: 134
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90275-0

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✦ Synopsis

The effect of sample vial type and sample composition on the Cerenkov count rate detected from 32P and 36Cl was studied using a liquid scintillation counter. When counting was done in the noncoincident mode, glass vials allowed higher counting efficiency than plastic vials. In the coincident mode light scattering caused by polyethylene and polyproplyene vials allowed higher counting efficiency than glass vials. Highest coincident counting efficiency was from plastic minivials in a glass carrier vial. Increased solute concentration in samples caused increased counting efficiency due to changes in the refractive index of the solution. This can cause significant counting efficiency changes with no sample channel ratio change in density gradient fractions. The use of wavelength shifters is shown to be inappropriate when the sample pH varies, as this can change the fluorescent properties of the shifters and thereby the observed count rate.

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