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The effect of rhGM-CSF on the proliferation of osteogenic sarcoma cells

✍ Scribed by Dr. W. Jeffrey Baker; Jeffrey B. Hargis; Romano Danesi; Renato V. La Rocca

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
400 KB
Volume
37
Category
Article
ISSN
0361-8609

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Recombinant human granulocyte–macrophage colony‐stimulating factor (rhGM‐CSF) promotes the growth of a variety of hematopoietic and nonhematopoietic cells, both benign and malignant. There is now evidence that osteoblast‐like cells produce GM‐CSF and their growth is stimulated by this cytokine in vitro. We have studied the effect of rhGM‐CSF on DNA synthesis and cell proliferation in the human osteogenic sarcoma cell lines U‐20S, G‐292, MG‐63, and HOS. RhGM‐CSF stimulated a dose‐dependent increase in radioactive thymidine incorporation in each of the four cell lines in the presence of serum‐free media, and in two cell lines (HOS and U‐20S) in the presence of fetal bovine serum (FBS). In addition, rhGM‐CSF produced significant increases in cell proliferation in two cell lines (MG‐63 and U‐20S) in the presence of 2% FBS. These results suggest that GM‐CSF may have an important role in the biology of human osteogenic sarcoma cells. The clinical implications of these findings merit further investigation.

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