## Abstract ## Background Efficient delivery and expression of plasmids (pDNA) is a major concern in gene therapy and DNA vaccination using nonβviral vectors. Besides the use of adjuvants, the pDNA vector itself can be designed to maximize survival in nucleaseβrich environments. Homopurineβrich tr
The effect of presumptive polyadenylation signals on the expression of the CAT gene in transgenic tobacco
β Scribed by Georgina Hernandez; Frank Cannon; Maura Cannon
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 382 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0721-7714
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Deletions were made in the cauliflower mosaic virus polyadenylation sequence which was cloned downstream of the beta-glucuronidase gene (gus). The populations of mRNAs generated in tobacco mesophyll protoplasts by transient expression with the various constructs were analysed using a polymerase chai
Homospermidine synthase (HSS) is a branch-point enzyme that links the secondary pathway (pyrrolizidine alkaloids) to primary metabolism (polyamines). Since the diamine putrescine is a precursor of homospermidine and nicotine in tobacco, we performed heterologous expression of a bacterial homospermid
A genomic clone containing the maize __Cat1__ gene has been isolated and its complete DNA sequence determined. The start of transcription has been mapped by primer extension. Six introns were identified in the __Cat1__ coding region. In order to determine the tissueβspecific expression pattern of th