𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The effect of phytohemagglutinin on rat thymus cellsin vitro

✍ Scribed by Schwarz, M. Roy ;Rieke, William O.

Publisher
John Wiley and Sons
Year
1966
Tongue
English
Weight
724 KB
Volume
155
Category
Article
ISSN
0003-276X

No coin nor oath required. For personal study only.

✦ Synopsis

Cells obtained from the thymus glands of five strains of rats, ranging in age from five hours to five months, were cultured in the presence of phytohemag glutinin according to the method of Rieke and Schwarz ('64). In these cultures, the large and medium lymphocytes in the inoculum gave rise by mitosis to small lymphocytes. Under the influence of PHA, small lymphocytes were transformed into enlarged cells which in turn acted as precursors of small lymphocytes. However, not all small ceUs in the cultures nor thymic lymphocytes from newborn rats responded to this mitogenic drug. A progressive increase in responsiveness was detected as donors increased from five hours to 32 days of age.

MATERIAL AND METHODS

  1. AnimaEs. Seventy-five male rats ranging in age from 5 hours to 20 weeks were employed in this study. While Lewis strain rats were utilized to prepare the majority of cultures, Brown Norway (Chocolate),

📜 SIMILAR VOLUMES

The effect of carcinogenic hydrocarbons
The effect of carcinogenic hydrocarbons on rodent and primate cellsin vitro
✍ Diamond, Leila 📂 Article 📅 1965 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 821 KB

The effect of polycyclic hydrocarbons on rodent and primate cells in uitro was studied using dimethyl sulfoxide as the initial solvent for the hydrocarbons prior to their addition to the cell cultures. Because the dimethyl sulfoxide was not toxic to the cells at a final concentration in which relati

Effect of metal ions on the formation an
Effect of metal ions on the formation and function of osteoclastic cellsin vitro
✍ Nichols, K. G. ;Puleo, D. A. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 112 KB 👁 2 views

To determine if metal ions play a contributing role in loosening of orthopedic implants, the present work investigated whether sublethal concentrations of ions affect the formation and function of osteoclasts in vitro. Rat bone marrow cells were cultured on slices of devitalized bone and in the pres

Effects of patulin on thymus capillary o
Effects of patulin on thymus capillary of rats
✍ Nursel Gül; Nesrin Özsoy; Özlem Osmanagaoglu; Güldeniz Selmanoğlu; E. Arzu Koçka 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 330 KB 👁 2 views
Dose-dependent effects of carbendazim on
Dose-dependent effects of carbendazim on rat thymus
✍ Sibel Hayretdağ Songür; E.Arzu Koçkaya; Güldeniz Selmanoĝlu; Nurhayat Barlas 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 168 KB 👁 2 views
Effect of patulin on the interdigitating
Effect of patulin on the interdigitating dendritic cells (IDCs) of rat thymus
✍ Nesrin Özsoy; Güldeniz Selmanoğlu; E. Arzu Koçkaya; Nursel Gül; Suna Cebesoy 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 178 KB 👁 2 views

## Abstract Patulin is a common fungal contaminant of ripe apples used for the production of apple juice concentrates and it is also present in other fruits, vegetables and food products. Patulin is a secondary metabolite produced by species of the genera Penicillium, Aspergillus and Byssochlamys.

Effect of hormones and growth factors on
Effect of hormones and growth factors on the proliferation of adult cricket neural progenitor cellsin vitro
✍ Malaterre, Jordane ;Strambi, Colette ;Aouane, A�cha ;Strambi, Alain ;Rougon, Gen 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 519 KB

In the adult cricket brain, a cluster of neuroblasts produces new interneurons that integrate into the mushroom body (MB), the main associative structure for multisensory information of the insect brain. In previous study we showed the antagonist role of the two morphogenetic hormones, juvenile horm