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The effect of phytohemagglutinin on glucose-U-C14 oxidation by peritoneal and blood white cells of the rat

✍ Scribed by Josef P. Hrachovec

Publisher: John Wiley and Sons
Year: 1966
Tongue: English
Weight: 523 KB
Volume: 67
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040670305

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✦ Synopsis

Incubation of washed white cells with phytohemagglutinin (PHA) had a stimulatory effect on the rate of C"OZ evolution from uniformly C14-labeled glucose by the cells. The intensity of this effect depended not only on the concentration of PHA, but also on the duration of soaking of the cells in protein-free medium for tissue culture before PHA was added and on the length of incubation of the soaked cells with PHA before g1uco~e-U-C~~ was added. This effect of PHA on the rate of glucose-U-Ci4 oxidation by the cells was essentially the same, whether white blood cells or peritoneal white cells were used.

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