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The effect of oxygen tension on proteoglycan synthesis and aggregation in mammalian growth plate chondrocytes

โœ Scribed by Dr. Charles C. Clark; Brad S. Tolin; Carl T. Brighton

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
893 KB
Volume
9
Category
Article
ISSN
0736-0266

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โœฆ Synopsis

Abstract

Growth plate chondrocytes isolated from the proliferative and hypertrophic zones of bovine costochondral junctions were grown in vitro in the presence of various oxygen tensions ranging from 3 to 60%. Using [^35^S] sulfate as an index of glycosaminoglycan synthesis, incorporation was found to be maximal at 21% O~2~. In contrast, proteoglycan aggregation under the same conditions was found to be maximal at 3% O~2~. There were no consistent differences in response between cells from the different morphologic zones even though they are exposed to different oxygen tension in situ. These results show that proteoglycan synthesis and aggregation in growth plate chondrocytes in vitro are differentially affected by the ambient oxygen environment.

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