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The Effect of O2 Tension on the Blastocyst (BL) Development of Mouse Embryos In Vitro

✍ Scribed by T Mukaida; K Takahashi; Y Yamauchi; T Horiuchi

Book ID
119416511
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
56 KB
Volume
74
Category
Article
ISSN
1556-5653

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## Abstract The effect of inhibitors of RNA synthesis (Cordycepin, Actinomycin D) and protein synthesis (Cycloheximide) on the development and growth of mouse blastocysts in vitro was explored. Blastocysts exposed in vitro for 24 hours to 50 ΞΌg/ml Cordycepin, 0.005 ΞΌg/ml Actinomycin D, or 0.1 ΞΌg/ml

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## Abstract The optimal oxygen tension for development of preimplantation mouse embryos to the blastocyst stage in vitro was found to be between 2.5% and 5%. One‐ and two‐cell embryos had a more sharply defined range of oxygen tension capable of supporting development than 8‐cell and morula stages.