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The effect of nordihydroguaiaretic acid on iodoacetate-induced toxicity in cultured neurons

✍ Scribed by Noemí Cárdenas-Rodríguez; Silvia Guzmán-Beltrán; Omar Noel Medina-Campos; Marisol Orozco-Ibarra; Lourdes Massieu; José Pedraza-Chaverri

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
166 KB
Volume
23
Category
Article
ISSN
1095-6670

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✦ Synopsis

Abstract

Nordihydroguaiaretic acid (NDGA) is present in high concentrations in the desert shrub Creosote bush, Larrea tridentate. This plant has been used in traditional medicine because of its beneficial effects related, at least in part, to its antioxidant properties. Taking into account some evidence about neuroprotective effects elicited by NDGA, we evaluated the effect of this compound on the neurotoxicity induced by iodoacetate (IAA), an inhibitor of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), on cerebellar granule neurons. In addition, as reactive oxygen species play an important role in IAA‐induced cytotoxicity, we also studied the enzymatic antioxidant system in IAA‐treated cells. We found that IAA caused a dose‐dependent decrease in cell viability of cultured neurons with an IC~50~ of 18.4 µM and induced increased activity of catalase, glutathione peroxidase, and glutathione‐S‐transferase. Moreover, NDGA attenuated the toxicity induced by 18.4, 25, and 30 µM of IAA without abolishing the inhibitory effect of IAA on GAPDH activity. Furthermore, NDGA could prevent the inhibitory effect of IAA on aconitase activity, a marker of oxidative stress, suggesting that the protective effect of NDGA on IAA neurotoxicity was associated with the prevention of oxidative stress. © 2009 Wiley Periodicals, Inc. J Biochem Mol Toxicol 23:137–142, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20278

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