The effect of nitrogen refeeding on the carbohydrate content into nitrogen-starved cells ofPlatymonas striataButcher

✍ Scribed by P.A. Edge; T. R. Ricketts

• Book ID: 104755437
• Publisher: Springer-Verlag
• Year: 1977
• Tongue: English
• Weight: 315 KB
• Volume: 136
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00396192

✦ Synopsis

Studies on the mean cellular carbohydrate contents of Platymonas striata Butcher under conditions of nitrogen-starvation, and after refeeding these starved cultures with either nitrate or ammonium ions (growing under continuous illumination or with an alternating light/dark regime) have shown that nitrogen-starved cells accumulated abnormal amounts of cellular carbohydrate and that nitrogen refeeding produced a marked drop in the cellular carbohydrate. Cells grown in a light/dark regime accumulated less carbohydrates than those grown in continuous light. The mean cellular carbohydrate levels 16 h after nitrogen refeeding were still much in excess of those of cells grown with normal nutrition. It was therefore suggested that the differences in nitrogen uptakes in this period-when comparing either the uptake of cells grown in continuous light with that of cells grown in a light/dark regime; or when comparing the uptakes of cells presented with either nitrate or ammonium ions and grown in a light/dark regimecannot be directly due to shortages of carbohydrate for the provision of carbon skeletons for nitrogen assimilation.