The effect of native and single stranded DNA on the platelet release reaction

✍ Scribed by Carole A. Dorsch; Jody Killmayer

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 596 KB
• Volume: 26
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780260209

✦ Synopsis

Abstract

Native (n) but not single stranded (ss) DNA was found to induce release of ^3^H‐serotonin (5‐HT) from platelets of the majority of normal individuals. However, ssDNA markedly enhanced 5‐HT release induced by heat‐aggregated IgG (aggIgG), while less enhancement was seen using nDNA. Similar enhancement was produced by polyinosinic acid but not by polyinosinic:polycytidylic acid. The ability of ssDNA to potentiate aggIgG‐induced 5‐HT release seemed specific for aggIgG, since no effect on ADP or epinephrine‐induced release was observed and thrombin‐induced release was inhibited. In contrast, nDNA in high concentrations (100 μg/ml) potentiated ADP, epinephrine, and thrombin‐induced 5‐HT release. These results suggest that ss‐and nDNA may interact with platelets by different mechanisms and provide a means by which DNA, released at sites of tissue injury, could modulate the role of platelets in the inflammatory response. The ability of DNA to enhance the aggIgG‐induced platelet release reaction may be important in immune complex diseases such as systemic lupus erythematosus.