The effect of morphine tolerance on the incorporation of 3H-leucine into proteins of rat synaptic membranes

✍ Scribed by Doris H. Clouet; Milton Ratner

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 625 KB
• Volume: 4
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490040203

✦ Synopsis

The rate of incorporation of 3H-leucine into proteins of the synaptic junctional and nonjunctional membranes and synaptic vesicles of rat brain has been examined in control and morphine-tolerant rats. There are no discernible differences between control and tolerant animals in amount of protein as measured by densitometric tracings of dye-stained proteins separated by acrylamide gel electrophoresis from the three membrane fractions of whole brain areas. However, there are differences in the turnover of membrane protein: three vesicle protein bands and one junctional-membrane protein band are significantly more highly labeled, and one junctional-membrane protein is significantly less highly labeled by 3H-leucine in samples from tolerant rats. Of these, the two junctional-membrane proteins can be tentatively identified as components of the post synaptic densities, and one of the vesicle proteins as tubulin.