The effect of metal ions on the alkaline phosphatase ofRhizobium leguminosarum
β Scribed by A. R. Glenn; M. J. Dilworth
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 461 KB
- Volume
- 126
- Category
- Article
- ISSN
- 0302-8933
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Alkaline phosphatase (EC 3.1.3.1) from bovine intestinal mucosa (BIAP) is an homodimeric metalloenzyme, containing one Mg 2Ψ and two Zn 2Ψ ions in each active site. ApoBIAP, prepared using ion-chelating agents, exhibited a dramatic decrease of its hydrolase activity, concomittant to conformational c
Inhibition of immobilized alkaline phosphatase by several metal ions and EDTA was studied in a single-channel flow-injection system. The enzyme was immobilised on controlled pore glass. The same enzyme preparation was used for three months. The inhibition by the tested compounds is reversible, the i