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The effect of mercuric ion on the DNA–methylene blue complex

✍ Scribed by Bhabatarak Bhattacharya; Nanibhushan Datta Gupta; Uma Shankar Nandi


Publisher
Wiley (John Wiley & Sons)
Year
1971
Tongue
English
Weight
191 KB
Volume
10
Category
Article
ISSN
0006-3525

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✦ Synopsis


2625 T h e Eflect of Mercuric Ion on the DN-4-Methylene Blue Complex

Mercuric ions have been shown to react specifically with dye-nucleic acids complexes1

But the actual nature of binding of In the present communica-Calf thymus DNA supplied by B. I>. H., Pool, England, has been used without fur-D S A solution was prepared in sodium cacodylate buffer (1 x 10-3 moles/liter) and pH was maintained Jlethylene blue (AZB) as zinc salt (E. AIerck, Germany) was recrystal-JIercuric chloride, ((2. It., I:. hlerck, Germany) has been used as and are able to lower dye binding of nucleic acids.2 mercuric ion to IINA in presence of dye is not yet settled. tion effects of mercuric ions on DNA-methylene blue complex have been presented.


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