The effect of laminin peptide gradient in enzymatically cross-linked collagen scaffolds on neurite growth
✍ Scribed by Li Yao; Gopinath Damodaran; Natalia Nikolskaya; Adrienne M. Gorman; Anthony Windebank; Abhay Pandit
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 575 KB
- Volume
- 9999A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
Guided neurite growth is critical in both peripheral nervous system and central nervous system nerve regeneration. Scaffolds that provide structural and guidance cues for neuronal cells have a potential role in neural regeneration application. Type I collagen is suitable to be processed as an engineered scaffold for nerve regeneration because of its biological and structural properties. A few previous studies have shown that cross‐linking of collagen scaffolds with microbial transglutaminase improves the mechanical strength and degradation properties of the scaffolds. It was shown that laminin 5 can regulate neurite outgrowth and extension. A motif (PPFLMLLKGSTR) in the human laminin 5 α3 chain is crucial for both integrin α3β1 receptor binding and cell adhesion. In the present work, we studied the guidance effect of a laminin peptide (PPFLMLLKGSTR) gradient in collagen and cross‐linked collagen scaffolds on neurite growth. Neurites of rat pheochromocytoma (PC12) cells showed a preferential growth toward the high laminin concentration level on the collagen scaffold, while the incorporation of laminin peptide in the scaffold did not influence neurite length of PC12 cells. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010