The effect of K+/H+ antiporter nigericin on gap junction permeability

✍ Scribed by Irene V. Budunova; Leonid A. Mittelman

• Book ID: 104628513
• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 599 KB
• Volume: 8
• Category: Article
• ISSN: 0742-2091
• DOI: 10.1007/bf00119295

✦ Synopsis

The K+/H + antiporter nigericin inhibits the intercellular exchange of the fluorescent dye Lucifer Yellow between DM15-transformed fibroblasts derived from the Djungarian hamster. The efficacy of nigericin action was related to its concentration and time of incubation. The nigericin-induced uncoupling effect on gap junctions was reversible and was shown to be based on its ability to cause cystolic acidification. The effect of nigericin on dye-coupling in intact and 12-0tetradecanoyl-phorbol-13-acetate (TPA)-pretreated cells did not differ, indicating that the uncoupling effect of H + on gap junctions in DM15 cells was not mediated by the TPA-dependent isoform of protein kinase Co