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The effect of intracellular pH on the regulation of the Rab 16A and the α-amylase 1/6-4 promoter by abscisic acid and gibberellia

✍ Scribed by S. Heimovaara-Dijkstra; J. Mundy; M. Wang

Publisher: Springer
Year: 1995
Tongue: English
Weight: 514 KB
Volume: 27
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00020234

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✦ Synopsis

Intracellular pH (pHi) of barley aleurone cells is known to be affected by hormones and plant growth conditions. The possible mechanisms by which these pH i shifts influence the actions of abscisic acid (ABA) or gibberellin (GA) is being investigated. Here we report an attempt to study the effect of pHi on hormone-induced gene expression. We used weak acids and weak bases to artificially mimic the pHi changes brought about by ABA and GA and found that chloramphenicol acetyltransferase (CAT) expression controlled by the Rab promoter was affected while the e-amylase promoter seemed insensitive. CAT fused to the 35S promoter was used as a control which is not inducible by ABA or GA3. The expression of this construct was not significantly affected by artificial pHi changes.

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