The Effect of Inhaled Heparin and Related Glycosaminoglycans on Allergen-induced Eosinophil Infiltration in Guinea-Pigs

✍ Scribed by E.A.M. Seeds; A.P. Horne; D.J. Tyrrell; C.P. Page

• Publisher: Elsevier
• Year: 1995
• Tongue: English
• Weight: 598 KB
• Volume: 8
• Category: Article
• ISSN: 0952-0600
• DOI: 10.1006/pulp.1995.1012

✦ Synopsis

SUMMARY: Aerosolized unfractionated heparin GM1060 significantly inhibited allergen-induced eosinophil infiltration into the airways of guinea-pigs (as assessed both histologically and by bronchoalveolar lavage, or BAL) at doses of 160 and (1600 \mathrm{U} / \mathrm{ml}). Similarly aerosolized unfractionated heparin, Multiparin was effective at reducing eosinophil levels in the BAL fluid at 1000,2000 and (5000 \mathrm{U} / \mathrm{ml}), but this reduction was statistically significant only at the highest dose used.

Additionally, Fragmin (a low molecular weight heparin) significantly inhibited allergen-induced eosinophil infiltration into BAL fluid at a dose of (500 \mathrm{U} / \mathrm{ml}), an effect that was lost at the higher doses of 1000 and (2000 \mathrm{U} / \mathrm{ml}).

Allergen-induced eosinophil infiltration was unaffected by dermatan sulphate. However, the glycosaminoglycans chondroitin sulphate (A), chondroitin sulphate (C) and heparan sulphate were able to influence the extent of allergeninduced eosinophil infiltration into BAL fluid.

These results suggest that heparin and some related glycosaminoglycans can inhibit allergen-induced eosinophil infiltration when administered directly to the airways.