The effect of handling methods on subsequent plasma progesterone levels in sheep

✍ Scribed by E. Mukasa-Mugerwa; T. Bekele; O. B. Kasali

• Book ID: 104742641
• Publisher: Springer Netherlands
• Year: 1989
• Tongue: English
• Weight: 310 KB
• Volume: 13
• Category: Article
• ISSN: 0165-7380
• DOI: 10.1007/bf00366855

✦ Synopsis

Mukasa-Mugerwa, EL, Bekelye, T. and Kasali, O.B. 1989. The effect of handling methods on subsequent plasma progesterone levels in sheep. Vererimny Research Communicafions, l3 (l), 75-79

The mean progesterone concentration in the plasma of 10 adult Ethiopian Highland sheep obtained immediately after slaughter was 10.56 2 3.98 rig/ml. Samples were subsequently incubated at 4"C, room temperature (19-22°C) or 26°C as either plasma or intact but c&rated blood. Failure to separate plasma affected the progesterone content at 2-72 h at room temperature or 26°C (p-zO.01 -p<O.OOOl). Incubation temperature affected the plasma concentration at 18 h (pcO.05) and 24 h (p<O.OOl). Although progesterone values were generally higher in separated plasma, disparity with the values from plasma separated from incubated titrated blood was small (r=O.76-0.98). Progesterone concentration declined haphazardly after collection but sometimes exceeded the initial readings. This kept the average concentration of progesterone in plasma separated immediately after collection fairly constant and within 15% of zero time samples during the first 48 h.