The Effect of Folding Catalysts on the In Vivo Folding Process of Different Antibody Fragments Expressed in Escherichia coli
✍ Scribed by Knappik, Achim ;Krebber, Claus ;Plückthun, Andreas
- Book ID
- 109901228
- Publisher
- Nature Publishing Group
- Year
- 1993
- Tongue
- English
- Weight
- 1007 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1087-0156
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Trifluoroethanol (TFE) has been used to probe differences in the stability of the native state and in the folding pathways of the homologous cysteine protein inhibitors, human stefin A and B. After complete unfolding in 4.5 mol/L GuHCl, stefin A refolded in 11% (vol/vol) TFE, 0.75 mol/L GuHCl, at pH
## Abstract High‐performance tangential flow filtration (HPTFF) is shown to successfully enable concentration, purification and formulation in a single unit operation. This is illustrated with feedstreams comprising recombinant proteins expressed in __Escherichia coli__ (__E. coli__). Using positiv