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The effect of DNA replication on mutation of theSaccharomyces cerevisiae CDC8gene

✍ Scribed by D. Zaborowska; J. Zuk

Publisher
Springer-Verlag
Year
1990
Tongue
English
Weight
493 KB
Volume
17
Category
Article
ISSN
0172-8083

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✦ Synopsis

Incubation in YPD medium under permissive conditions when DNA replication is going on, strongly stimulates the induction of cdc+ colonies of UV-irradiated cells of yeast strains HB23 (cdc8-1/cdc8-3), HB26 (cdc8-3/cdc8-3) and HB7 (cdc8-1/cdc8-1). Inhibition of DNA replication by hydroxyurea, araCMP, cycloheximide or caffeine or else by incubation in phosphate buffer pH 7.0, abolishes this stimulation. Thus the replication of DNA is strongly correlated with the high induction of cdc+ colonies by UV irradiation. It is postulated that these UV-induced cdc+ colonies arise as the result infidelity in DNA replication.

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