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The effect of different solvents on the ATP/ADP content and growth properties of HeLa cells

✍ Scribed by Stanislav Forman; Jan Káˇs; Fabiana Fini; Mark Steinberg; Tomáˇs Ruml

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
162 KB
Volume
13
Category
Article
ISSN
1095-6670

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✦ Synopsis

Testing of the effects of xenobiotics in cultured cells often requires the use of organic solvents to effect suspension of the test agents in cell culture media. However, the toxic effects of the solvents themselves may introduce artifacts, which obscure interpretation of the experimental results. In this article, the toxicity of different solvents commonly used for solvation of a variety of xenobiotic agents was studied. We show that ethanol, acetone, isooctane, methanol, and hexane were considerably less toxic than the more commonly used solvent, DMSO, when ATP content and growth rates of HeLa cells exposed to these solvents was measured.

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