The effect of combined application of TGFβ-1, BMP-2, and COLLOSS® E on the development of bone marrow derived osteoblast-like cells in vitro
✍ Scribed by M. van der Zande; X. F. Walboomers; A. Briest; M. Springer; J. I. Álava; J. A. Jansen
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 335 KB
- Volume
- 86A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
This study investigated the combined application of Transforming Growth Factor β‐1 (TGFβ‐1) and Bone Morphogenetic Protein‐2 (BMP‐2) to stimulate osteogenic expression in vitro. TGFβ‐1 and BMP‐2 fulfill specific roles in the formation of new bone. COLLOSS® E, a bone‐derived collagen product containing a variety of naturally occurring growth factors, was also used. Growth factors were administered to osteoblast‐like cells from rat bone marrow (RBM). Proliferation and differentiation were monitored up to 24 days, by measuring total DNA content, alkaline phosphatase activity, and calcium content. Genetic expression of a set of differentiation markers at day 7 was measured by Q‐PCR. Adding BMP‐2 alone induced high proliferation rates, compared to the growth factor supplemented groups, and it induced high differentiation rates, compared to the control group. Adding TGFβ‐1 combined with BMP‐2, TGFβ‐1 alone, or COLLOSS® E resulted in a significant decrease in proliferation rate, but an increase in differentiation rate, compared to the control group. Additive or synergistic effects of application of TGFβ‐1 and BMP‐2 were not observed. The observed effects of COLLOSS® E mainly resembled those of TGFβ‐1 application alone. It can be concluded that BMP‐2 is the most suitable candidate for osteogenic stimulation of RBM cells in these settings. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008