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The effect of calcium on the initiation and growth of human epidermal cells

✍ Scribed by P. J. Dykes; L. A. Jenner; R. Marks

Book ID
104762062
Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
349 KB
Volume
273-273
Category
Article
ISSN
0340-3696

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✦ Synopsis

The effect of the concentration of Ca 2 + in the medium on the plating efficiency of human epidermal cells has been investigated. Primary cultures plated onto lethally irradiated mouse embryo fibroblasts showed not only increased colony formation at lowered (0.2 mM) Ca 2 + levels but also increased growth rate as indicated by greater colony size. On the other hand, secondary cultures showed little variation in plating efficiency and only small differences in colony size were apparent over the range 0.2-1.64 mM Ca 2+. Epidermal growth factor had no effect on the Ca 2 + dependence of primary or the Ca 2 + independence of secondary cultures.

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