The effect of caffeine on fragile X expression
β Scribed by M. A. Abruzzo; Dorothy Pettay; Martha Mayer; Patricia A. Jacobs
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 303 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0340-6717
No coin nor oath required. For personal study only.
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To enhance the cytogenetic expression of the fragile X chromosome, we studied the effects of hyperoxia and caffeine on the induction of fragile Xq27.3. A lymphoblastoid cell line (GM 06912) derived from a fragile X male proband was cultured in RPMI 1640 containing 16% dialyzed fetal calf serum. The
The fragile (X) chromosome demonstrable in individuals with one type of X-linked mental retardation is seldom, if ever, seen in more than 50% of cells of affected individuals. We have devised a model to explain this apparent 50% maximum, one essential feature of which is that the fragile (X) will no
The probability of a heterozygote being affected was estimated from the distribution of frequencies of early-replicating fragile X [fra(X)I chromosome in normal and mentally retarded heterozygotes, taking into account the prior probabilities of 0.35 for mental retardation and 0.65 for normality. The