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The effect of anisosmotic conditions on the morphology of the metaphase II spindle of human oocytes, and its relevance to cryopreservation

โœ Scribed by Steven F Mullen; Diane C Broermann; Claudia L Jenkins; Chad A Johnson; John K Critser


Book ID
119419256
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
18 KB
Volume
78
Category
Article
ISSN
1556-5653

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Effect of osmotic stress on the developm
โœ Y. Agca; J. Liu; J.J. Rutledge; E.S. Critser; J.K. Critser ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 143 KB ๐Ÿ‘ 1 views

The effects of osmotic stress on germinal vesicle (GV) and metaphase II (MII) stage bovine cumulus oocyte complexes (COCs) were evaluated by first exposing them to various anisotonic NaCl solutions (75, 150, 600, 1200, 2400, and 4800 ฯฎ 5 mOsm/kg) for 10 min and then returning them to isotonic TL-Hep