N-linked oligosaccharides in glycoproteins are considerably more stable to mild alkali than are O-linked chains has been reexamined. A number of 'H-labeled model glycoproteins (fetuin, transferrin, and glycophorin) were treated with: (i) 0.05 M OH--l M BH; at 50Β°C for 16 h, (ii) 0.1 M OH--O.8 M BH,
The effect of alkaline borohydride treatment onN-linked carbohydrates of glycoproteins
β Scribed by Sulabha P Argade; G Doyle Daves; Herman Van Halbeek; Jack A Alhadeff
- Book ID
- 105048570
- Publisher
- Springer US
- Year
- 1989
- Tongue
- English
- Weight
- 615 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1573-4986
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ABSTRACr A new procedure for the quantification by g.1.c. of the carbohydrate constituents of glycoproteins is proposed which involves (a) simultaneous action of neuraminidase and neuraminic acid aldolase, (b) hydrolysis with 4~ trifluoroacetic acid at 125" for 1 h, and (c) conversion of the product
Carbohydrate Chemistry and Glycobiology have witnessed a rapid expansion during the last few years with the development of numerous new, imaginative and efficient syntheses which provide further insight into structures and biological interactions of glycoconjugates. Glycosylation reactions are widel