✦ LIBER ✦

The Effect In vitro of High-Density Lipoprotein from Healthy and Infected Humans on the Oxidative Metabolism of Polymorphonuclear Leukocytes

✍ Scribed by CRISTIANE FURLANETO; ANA CAMPA

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 141 KB
Volume: 15
Category: Article
ISSN: 0263-6484
DOI: 10.1002/(sici)1099-0844(199703)15:1<39::aid-cbf718>3.0.co;2-d

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✦ Synopsis

We studied the effects in vitro of high-density lipoprotein from healthy (N-HDL) and from infected humans (AP-HDL) on the oxidative metabolism of human polymorphonuclear leukocytes (PMN). Products of the H2O2-MPO-halide system were monitored by luminol-enhanced chemiluminescence and superoxide anion formation was monitored by lucigenin-enhanced chemiluminescence during stimulation of human PMN with phorbol myristate acetate (PMA) or an opsonized stimulus (OS). The results showed that N-HDL and AP-HDL affect the oxidative metabolism of PMN in different ways. The possible role of this effect is discussed.

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