The dry-packing of columns in liquid chromatography

✍ Scribed by Davies, R. D.

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 538 KB
• Volume: 4
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240040605

✦ Synopsis

Abstract

This contribution comprises two parts, the one covering some variables in the dry‐packing of columns for liquid chromatography, the other the use of an automatic dry‐packer in liquid chromatography. A high‐pressure stainless steel column was dry‐packed using a 20‐44 μm size, irrregularly shaped, silica gel support. It was found that, when support was added in discrete amounts, lateral tapping produced a column of higher efficiency (HETP = 0.69 mm) than was the case for vertical tapping (HETP = 1.37 mm). Simultaneous vertical and lateral tapping produced an intermediate result. Bulk filling of the column followed by tapping was inferior to adding support in discrete amounts. An apparatus constructed to perform the vertical and lateral tapping operations automatically confirmed the above conclusions. It was concluded that commercially available dry‐packing apparatus do not offer the best action for packing an efficient column since they involve predominantly vertical tapping.