The Drosophila ACP65A cuticle gene: Deletion scanning analysis of cis-regulatory sequences and regulation by DHR38
✍ Scribed by N. Bruey-Sedano; J. Alabouvette; M. Lestradet; L. Hong; A. Girard; E. Gervasio; B. Quennedey; J.P. Charles
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 796 KB
- Volume
- 43
- Category
- Article
- ISSN
- 1526-954X
No coin nor oath required. For personal study only.
✦ Synopsis
The regulatory sequences of the Drosophila ACP65A cuticle gene were analyzed in vivo in transgenic flies, using both fusion genes constructs and transposase-mediated deletions within a P element containing ACP65A regulatory sequences fused to the lacZ gene (deletion scanning). The sequences located between À594 and þ161 are sufficient to confer both temporal and spatial expression specificities, indicating the presence of tissue-specific enhancers and response elements to hormone-induced factors. In addition, timing of expression and tissue-specificity appear to be controlled by distinct cis-regulatory elements, which suggests the existence of independent hormonal and tissue-specific signaling pathways. Gain and loss of function studies also implicate DHR38, the Drosophila homolog of the vertebrate NGFI-B-type nuclear receptors, as an important activator of the ACP65A gene. genesis 43:17-27, 2005. V