We have examined DNA strand breakage, DNA degradation, and the rate of DNA synthesis in lig and lig-recB strains of Escherichia coli K12 incubated in the presence and absence of 3 mug/ml chloramphenicol. Substantial DNA strand breakage and DNA degradation is observed in the lig strain upon growth at
The DNA repair capability ofcdc9, theSaccharomyces derevisiaemutant defective in DNA ligase
โ Scribed by Leland H. Johnston
- Book ID
- 104693668
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 343 KB
- Volume
- 170
- Category
- Article
- ISSN
- 1617-4615
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โฆ Synopsis
The cell cycle mutant, cdc9, in the yeast Saccharomyces cerevisiae is defective in DNA ligase be deficient in the repair of DNA damaged by methyl methane sulphonate. On the other hand survival of cdc9 after irradiation by gamma-rays is little different from that of the wild-type, even after a period of stress at the restrictive temperature. The mutant cdc9 is not allelic with any known rad or mms mutants.
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