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The distinctive functions of the two structural calcium atoms in bovine pancreatic deoxyribonuclease

✍ Scribed by Ching-Ying Chen; Shao-Chun Lu; Ta-Hsiu Liao

Book ID
111753271
Publisher
Cold Spring Harbor Laboratory Press
Year
2009
Tongue
English
Weight
330 KB
Volume
11
Category
Article
ISSN
0961-8368

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The Tyr92-Pro93 peptide group of bovine pancreatic ribonuclease A (RNase A) exists in the cis conformation in the native state. From unfolding/refolding kinetic studies of the disulfide-intact wild-type protein and of a variant in which Pro93 had been replaced by Ala, it had been suggested that the