The differential expression of H-2K versus H-2D antigens, distinguishing high- metastatic from low- metastatic clones, is correlated with the immunogenic properties of the tumor cells
✍ Scribed by Lea Eisenbach; Nurit Hollander; Lea Greenfeld; Hadas Yakor; Shraga Segal; Michael Feldman
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- French
- Weight
- 700 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Two clones of the 3LL Lewis lung carcinoma, a lowmetastatic clone A9 and a high-metastatic clone DIZZ, were studied for MHC expression and immunogenic p r o p erties. Using monoclonal antibodies, we demonstrated that the A9 clone expresses both the H-2Kb and the H-2Db.
whereas the D I22 expresses only the H-ZD ', and lacks the expression of the H-2Kb encoded molecules. Cells of the low-metastatic clone A9 grew progressively in syngeneic (C57BU6J) or in F, mice, but were rejected in allogeneic recipients. The high-metastatic D I22 grew progressively in all mouse strains tested, yet metastases were formed only in syngeneic recipients. When H-2 recombinant mice were used, the A9 again manifested a significantly greater immunogenic potency than the metastatic DI 22, which grew in all 4 recombinants tested. Metastases, however, were formed in B I OHTG and to a lesser extent in B IOA(4R), thus indicating that metastasis formation is restricted by both C57BL background and H-ZD sub region. We subsequently tested whether the higher immunogenicity of the H-2Kb-positive A9 cells is expressed also in syngeneic mice, to examine whether this could account for its low metastatic phenotype. We found that immunization by A9 cells significantly inhibited the growth of a subsequent A9 graft and even of 0122, yet D122 did not retard the growth of secondary Dl22 or A9 cells. The increased immunogenic effect was expressed also in the generation of syngeneic cytotoxic lymphocytes by A9 but not by DlZZ cells. We suggest that expression of H-2K molecules on the 3LL clones, immunogenicity and the metastatic phenotype are causally related in this system.