The differential effect of Ca2+and Mg2+on the ATPase activity of cardiac myofibrils
β Scribed by H. Rupp
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 76 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1432-1017
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The Ca(2+)-activated myosin-ATPase and its dependence on hypoxia were assessed in freshwater turtle, rainbow trout, and in some cases rat. At 20 degrees C and pH 7.3, the maximal ATPase activity was (mean +/- SEM): turtle 0.040 +/- 0.003, trout 0.090 +/- 0.005, and rat 0.12 +/- 0.004 mmol\*min-1\*g-
## Abstract Crosslinking of membrane proteins of Escherichia coli with dithiobis (succinimidyl propionate) (DSP) resulted in loss of several enzyme activities including the Ca^2+^, Mg^2+^βactivated ATPase. This enzyme was crosslinked by DSP to the membrane and was not released by dialysis at low io