The determination of terbutaline in human plasma by selected ion monitoring of the t-butyldimethylsilyl ether

✍ Scribed by L. E. Martin; Janet Oxford; R. J. N. Tanner; M. J. Hetheridge

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 175 KB
• Volume: 6
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200061011

✦ Synopsis

A method is described for the quantitative determination of terbutaline in 2 ml human plasma. The drug is extracted from plasma as the terbutaline tetraphenylboron ion pair and determined by gas chromatography mass spectrometry of its t-butyldimethylsily ether. Salbutamol is used as internal standard. Quantification is achieved by selected ion monitoring of the ion m/z 482 derived from t-butyldimethylsilyl terbutaline and m/z 495 from t-butyldimethylsilyl salbutamol. The detection limit was estimated to be 250 pg terbutaline ml-1 plasma. The coefficient of variation at the level of 1 ng terbutaline ml-1 was 4.1% (n = 5).