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The determination of specific radioactivity of proteins eluted intact from polyacrylamide gels, utilizing a fluorescamine assay

✍ Scribed by Laura L. Kilgore; Julie L. Rogers; Bruce W. Patterson; Nancy H. Miller; Waldo R. Fisher


Book ID
107712205
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
709 KB
Volume
145
Category
Article
ISSN
0003-2697

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Excellent separation of proteins may very often be achieved rapidly and quite simply by zone electrophoresis in polyacrylamide gels. A major disadvantage of this method is the insolubility of the resultant gel, with the consequent difficulties in measuring both the quantities of prot,ein in the diff