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The determination of sedimentation constants of proteins and viruses with the help of the Spinco preparative ultracentrifuge

โœ Scribed by Alfred Polson; Anne M. Linder

Book ID
115785356
Publisher
Elsevier Science
Year
1953
Weight
555 KB
Volume
11
Category
Article
ISSN
0006-3002

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Working with detergent-solubilized bacteriorhodopsin we have used a table top preparative centrifuge for determination of \(M_{\mathrm{r}}\) of membrane proteins by sedimentation equilibrium. We demonstrate the use of two new methods to measure protein concentration as a function of distance from ro