Glucose metabolism of human red blood cells was investigated using carbon-13 nuclear magnetic resonance spectroscopy under both oxygenated and nonoxygenated conditions. These results show that under oxygenated conditions reversal of 3-phosphoglyceraldehyde to glucose is in competition with its catab
The determination of intracellular sodium concentration in human red blood cells: Nuclear magnetic resonance measurements
โ Scribed by Amira Rottman; Haggai Gilboa; Yael Schechter; Brain L. Silver
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 413 KB
- Volume
- 201
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
The intracellular sodium concentration and intracellular volume of human red blood cells were determined from 23Na and 1H NMR spectra. It is shown that sodium dissolved in the intracellular water has a concentration higher than that previously published. The intracellular sodium concentration measured was 11.4 +/- 3.1 mM. A comparison of different NMR methods used to determine sodium concentration is given.
๐ SIMILAR VOLUMES
Changes of intracellular sodium concentrations are often an indication of disease or malfunction. In this work, shift reagent-aided 23Na NMR spectroscopic determination of intracellular sodium was adapted to measurements with perfused cells embedded in agarose gel threads. Ehrlich ascites tumor cell