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The determination of inorganic orthophosphate in biological systems

✍ Scribed by Atkinson, A.; Gatenby, A.D.; Lowe, A.G.

Book ID
120057747
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
528 KB
Volume
320
Category
Article
ISSN
0304-4165

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πŸ“œ SIMILAR VOLUMES

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Ficoll hampers the transfer of unreduced phosphomolybdic acid (PMA) from aqueous to organic phase when determining inorganic phosphate (PI) by the method of Sanui [(1974)Anal. Biochem. 60,489-5041. This reduction in the transfer can be overcome by changing the organic solvent and the acidity in the

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Marsh (1) has proposed a procedure for the estimation of inorganic orthophosphate (Pi) in the presence of ATP, which is purportedly designed to overcome errors resulting from molybdate-catalyzed hydrolysis of ATP (2). In a critique of methods currently used for the assay of Pi in the presence of lab