The determination of Cu, Zn, and Mn in subcellular rat liver fractions

✍ Scribed by J. Smeyers-Verbeke; C. May; P. Drochmans; D.L. Massart

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 523 KB
• Volume: 83
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90080-x

✦ Synopsis

An atomic absorption technique is used for the determination of Cu, Zn, and Mn in subceilular fractions of individual rat livers. In addition to the su~matant fluid a determination of the trace elements was aIso carried out in the rough and smooth microsomal subfractions. The results show that the concentration of Mn is highest in the nuclei and mitochondria and lowest in the supernatant, whereas, for Zn and 01, the highest concentrations are found in the supernatant. The supernatant fraction was subjected to gel filtration to determine the amount of binding of Cu and Zn with the protein fraction of the supernatant fluid. 746